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Anti-PPP1R1B Antibody
Catalog #: 144-02580
PPP1R1B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UD71 |
| Gene Id | 84152 |
| Gene Symbols | PPP1R1B |
| Protein Name / Synonyms | PPP1R1B, DARPP-32, DARPP32, protein phosphatase 1 regulatory subunit 1B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
18kDa/22kDa
Observed MW
32kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human PPP1R1B (NP_001229393.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of 293T cells, using PPP1R1B antibody (144-02580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 3s.
Expiration:
12 months from the date of shipment when stored properly.