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ISO 13485:2016 cGMP

Anti-PPP2R1A Antibody

Catalog #: 144-05799

PPP2R1A Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P30153
Gene Id
5518
Gene Symbols
PPP2R1A
Protein Name / Synonyms
PPP2R1A, MRD36, PP2A-Aalpha, PP2AA, PP2AAALPHA, PR65A, protein phosphatase 2 scaffold subunit Aalpha
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW
65kDa
Observed MW
66kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PPP2R1A (NP_055040.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PPP2R1A antibody (144-05799) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PPP2R1A Polyclonal Antibody (144-05799)

Immunofluorescence analysis of U2OS cells using PPP2R1A antibody (144-05799). Blue: DAPI for nuclear staining.
Immunofluorescence - PPP2R1A Polyclonal Antibody (144-05799)

Expiration:
12 months from the date of shipment when stored properly.