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Anti-PPP2R5C Antibody
Catalog #: 144-05480
PPP2R5C Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13362 |
| Gene Id | 5527 |
| Gene Symbols | PPP2R5C |
| Protein Name / Synonyms | PPP2R5C, B56G, PR61G, B56gamma, protein phosphatase 2 regulatory subunit B'gamma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
52kDa/56kDa/61kDa/62kDa/64kDa
Observed MW
71kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human PPP2R5C (NP_848702.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPP2R5C antibody (144-05480) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using PPP2R5C antibody (144-05480) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PPP2R5C antibody (144-05480) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using PPP2R5C antibody (144-05480) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.