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Anti-PPP4R1 Antibody
Catalog #: 144-08361
PPP4R1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q8TF05 |
| Gene Id | 9989 |
| Gene Symbols | PPP4R1 |
| Protein Name / Synonyms | PPP4R1, MEG1, PP4(Rmeg), PP4R1, protein phosphatase 4 regulatory subunit 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
105kDa/107kDa
Observed MW
130kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (144-08361) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody (144-08361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (144-08361) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (144-08361). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (144-08361) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.