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Anti-PRKAA1 Antibody
Catalog #: 144-01229
PRKAA1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13131 |
| Gene Id | 5562 |
| Gene Symbols | PRKAA1 |
| Protein Name / Synonyms | PRKAA1, AMPK, AMPKa1, protein kinase AMP-activated catalytic subunit alpha 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
64kDa/65kDa
Observed MW
65kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKAA1 (NP_006242.5).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRKAA1 antibody (144-01229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using PRKAA1 antibody (144-01229). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.