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Anti-PRKAA2 Antibody
Catalog #: 144-07339
PRKAA2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P54646 |
| Gene Id | 5563 |
| Gene Symbols | PRKAA2 |
| Protein Name / Synonyms | PRKAA2, AMPK, AMPK2, AMPKa2, PRKAA, protein kinase AMP-activated catalytic subunit alpha 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
62kDa
Observed MW
69kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 343-552 of human PRKAA2 (NP_006243.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRKAA2 antibody (144-07339) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunofluorescence analysis of A549 cells using PRKAA2 antibody (144-07339). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.