RayBiotech

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ISO 13485:2016 cGMP

Anti-PRKACB Antibody

Catalog #: 144-05324

PRKACB Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P22694
Gene Id
5567
Gene Symbols
PRKACB
Protein Name / Synonyms
PRKACB, PKA C-beta, PKACB, PKAC-beta, cAMP-dependent protein kinase catalytic subunit beta
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
29kDa/36kDa/39kDa/40kDa/41kDa/46kDa
Observed MW
45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human PRKACB (NP_002722.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of SW480 cells, using PRKACB antibody (144-05324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - PRKACB antibody (144-05324)

Immunohistochemistry of paraffin-embedded mouse lung using PRKACB antibody (144-05324) at dilution of 1:200 (40x lens).
Immunohistochemistry - PRKACB antibody (144-05324)

Immunofluorescence analysis of U2OS cells using PRKACB antibody (144-05324). Blue: DAPI for nuclear staining.
Immunofluorescence - PRKACB antibody (144-05324)

Expiration:
12 months from the date of shipment when stored properly.