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Anti-PRKAG1 Antibody
Catalog #: 144-07300
PRKAG1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P54619 |
| Gene Id | 5571 |
| Gene Symbols | PRKAG1 |
| Protein Name / Synonyms | PRKAG1, AMPKG, 5'-AMP-activated protein kinase subunit gamma-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
34kDa/37kDa/38kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRKAG1 antibody (144-07300) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human stomach using PRKAG1 antibody (144-07300) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRKAG1 antibody (144-07300) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PRKAG1 antibody (144-07300) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using PRKAG1 antibody (144-07300). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.