Anti-PRKDC Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P78527
Gene Id	5591
Gene Symbols	PRKDC
Protein Name / Synonyms	PRKDC, DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350, DNA-dependent protein kinase catalytic subunit
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

465kDa/469kDa

Observed MW

469kDa

Immunogen

A synthetic peptide of human PRKDC

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PRKDC antibody (144-07716) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PRKDC Polyclonal Antibody (144-07716)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRKDC antibody (144-07716) at dilution of 1:100 (40x lens).
Immunohistochemistry - PRKDC Polyclonal Antibody (144-07716)

Immunohistochemistry of paraffin-embedded human kidney cancer using PRKDC antibody (144-07716) at dilution of 1:100 (40x lens).
Immunohistochemistry - PRKDC Polyclonal Antibody (144-07716)

Immunohistochemistry of paraffin-embedded mouse liver using PRKDC antibody (144-07716) at dilution of 1:100 (40x lens).
Immunohistochemistry - PRKDC Polyclonal Antibody (144-07716)

Expiration:

12 months from the date of shipment when stored properly.