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Anti-PROM1 Antibody
Catalog #: 144-12711
PROM1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43490 |
| Gene Id | 8842 |
| Gene Symbols | PROM1 |
| Protein Name / Synonyms | PROM1, AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, prominin-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:3000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
92kDa/93kDa/94kDa/96kDa/97kDa
Observed MW
115kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human PROM1 (NP_006008.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PROM1 antibody (144-12711) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using PROM1 antibody (144-12711) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.