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Anti-PRPF3 Antibody
Catalog #: 144-05482
PRPF3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43395 |
| Gene Id | 9129 |
| Gene Symbols | PRPF3 |
| Protein Name / Synonyms | PRPF3, HPRP3, HPRP3P, PRP3, Prp3p, RP18, SNRNP90, pre-mRNA processing factor 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
31kDa/77kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PRPF3 antibody (144-05482) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 Antibody (144-05482) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRPF3 antibody (144-05482). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.