Anti-PSAP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P07602
Gene Id	5660
Gene Symbols	PSAP
Protein Name / Synonyms	PSAP, GLBA, SAP1, prosaposin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

58kDa

Observed MW

58kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-310 of human PSAP (NP_002769.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PSAP antibody (144-01819) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 5s.
Western blot - PSAP antibody (144-01819)

Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody (144-01819) at dilution of 1:200 (40x lens).
Immunohistochemistry - PSAP Antibody (144-01819)

Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody (144-01819) at dilution of 1:200 (40x lens).
Immunohistochemistry - PSAP Antibody (144-01819)

Immunohistochemistry of paraffin-embedded rat brain using PSAP Antibody (144-01819) at dilution of 1:200 (40x lens).
Immunohistochemistry - PSAP Antibody (144-01819)