PSMB1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P20618 |
| Gene Id | 5689 |
| Gene Symbols | PSMB1 |
| Protein Name / Synonyms | PSMB1, HC5, PMSB1, PSC5, proteasome subunit beta 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
26kDa
Observed MW
23kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 29-241 of human PSMB1 (NP_002784.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PSMB1 antibody (144-01043) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse heart using PSMB1 antibody (144-01043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PSMB1 antibody (144-01043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using PSMB1 antibody (144-01043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using PSMB1 antibody (144-01043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PSMB1 antibody (144-01043) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using PSMB1 antibody (144-01043). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.