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Anti-PSMB5 Antibody
Catalog #: 144-01975
PSMB5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P28074 |
| Gene Id | 5693 |
| Gene Symbols | PSMB5 |
| Protein Name / Synonyms | PSMB5, LMPX, MB1, X, proteasome subunit beta 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
17kDa/21kDa/28kDa
Observed MW
21kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human PSMB5 (NP_002788.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PSMB5 antibody (144-01975) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using PSMB5 antibody (144-01975) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using PSMB5 antibody (144-01975). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.