Anti-PSMB5 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P28074
Gene Id	5693
Gene Symbols	PSMB5
Protein Name / Synonyms	PSMB5 (C-term)
Clonality	Polyclonal
Preparation	This PSMB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the C-terminal region of human PSMB5.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.

Specificity

Other Names

PSMB5; LMPX; MB1; X; Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X

NCBI Accession #

NP_001124197.1;NP_001138404.1;NP_002788.1

Other Accession #

NP_001124197.1; NP_002788.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

28480

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	PSMB5 Antibody (C-term) (Cat# 102-12526) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PSMB5 antibody detected the PSMB5 protein (arrow).
	Western blot analysis of PSMB5 (arrow) using rabbit polyclonal PSMB5 Antibody (C-term) (Cat# 102-12526). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMB5 gene.
	PSMB5 Antibody (C-term) (102-12526)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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