Anti-PTK6 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13882
Gene Id	5753
Gene Symbols	PTK6
Protein Name / Synonyms	PTK6, BRK, protein-tyrosine kinase 6
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

14kDa/51kDa

Observed MW

60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 222-451 of human PTK6 (NP_005966.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PTK6 antibody (144-07497) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PTK6 antibody (144-07497)

Immunohistochemistry of paraffin-embedded human gastric cancer using PTK6 antibody (144-07497) at dilution of 1:100 (40x lens).
Immunohistochemistry - PTK6 antibody (144-07497)

Immunofluorescence analysis of A549 cells using PTK6 antibody (144-07497). Blue: DAPI for nuclear staining.
Immunofluorescence - PTK6 antibody (144-07497)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products