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Anti-PTPRC Antibody
Catalog #: 144-02115
PTPRC Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P08575 |
| Gene Id | 5788 |
| Gene Symbols | PTPRC |
| Protein Name / Synonyms | PTPRC, B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, receptor-type tyrosine-protein phosphatase C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
130kDa/147kDa
Observed MW
185kDa
Immunogen
A synthetic peptide of human PTPRC
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse thymus, using PTPRC antibody (144-02115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using PTPRC antibody (144-02115) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PTPRC antibody (144-02115) (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PTPRC antibody (144-02115) (40x lens).
Immunofluorescence analysis of Jurkat cells using PTPRC antibody (144-02115) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.