Anti-PYCARD Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9ULZ3
Gene Id	29108
Gene Symbols	PYCARD
Protein Name / Synonyms	PYCARD, ASC, CARD5, TMS, TMS-1, TMS1, PYD and CARD domain containing
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

15kDa/19kDa/21kDa

Observed MW

22kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-195 of human PYCARD (NP_037390.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PYCARD antibody (144-01170).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PYCARD antibody (144-01170)

Immunofluorescence analysis of HeLa cells using PYCARD antibody (144-01170).
Immunofluorescence - PYCARD antibody (144-01170)

Expiration:

12 months from the date of shipment when stored properly.

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