Anti-RAB5A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P20339
Gene Id	5868
Gene Symbols	RAB5A
Protein Name / Synonyms	RAB5A, RAB5, ras-related protein Rab-5A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

22kDa/23kDa

Observed MW

22kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5A (NP_004153.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RAB5A antibody (144-01180) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] RAB5A Polyclonal Antibody (144-01180)

Western blot analysis of extracts from normal (control) and RAB5A knockout (KO) HeLa cells, using RAB5A antibody (144-01180) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - [KO Validated] RAB5A Polyclonal Antibody (144-01180)

Immunofluorescence analysis of HeLa cells using RAB5A antibody (144-01180). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] RAB5A Polyclonal Antibody (144-01180)

Expiration:

12 months from the date of shipment when stored properly.

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