Anti-RACGAP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9H0H5
Gene Id	29127
Gene Symbols	RACGAP1
Protein Name / Synonyms	RACGAP1, CYK4, HsCYK-4, ID-GAP, MgcRacGAP, rac GTPase-activating protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

71kDa

Observed MW

82kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 353-632 of human RACGAP1 (NP_001119576.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RACGAP1 antibody (144-05298) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - RACGAP1 antibody (144-05298)

Immunofluorescence analysis of U2OS cells using RACGAP1 antibody (144-05298). Blue: DAPI for nuclear staining.
Immunofluorescence - RACGAP1 antibody (144-05298)

Expiration:

12 months from the date of shipment when stored properly.

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