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Anti-RAD50 Antibody
Catalog #: 144-03078
RAD50 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q92878 |
| Gene Id | 10111 |
| Gene Symbols | RAD50 |
| Protein Name / Synonyms | RAD50, NBSLD, RAD502, hRad50, DNA repair protein RAD50 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Dilutions
WB 1:500 - 1:2000
IP 1:20 - 1:50
IP 1:20 - 1:50
Format
Calculated MW
138kDa/153kDa/154kDa
Observed MW
170kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAD50 (NP_005723.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RAD50 antibody (144-03078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug RAD50 antibody (144-03078). Western blot was performed from the immunoprecipitate using RAD50 antibody (144-03078) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.