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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q92878 |
| Gene Id | 10111 |
| Gene Symbols | RAD50 |
| Protein Name / Synonyms | RAD50, NBSLD, RAD502, hRad50, DNA repair protein RAD50 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
138kDa/153kDa/154kDa
Observed MW
180kDa
Immunogen
A synthetic peptide of human RAD50
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RAD50 antibody (144-60013) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human uterine cancer using RAD50 Antibody (144-60013) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody (144-60013) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using RAD50 Antibody (144-60013) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RAD50 Antibody (144-60013) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody (144-60013) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
