Anti-RAD9A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	Q99638
Gene Id	5883
Gene Symbols	RAD9A
Protein Name / Synonyms	RAD9A, RAD9, RAD9 checkpoint clamp component A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

42kDa

Observed MW

27kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse testis, using RAD9A antibody (144-01890) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - RAD9A Polyclonal Antibody (144-01890)

Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (144-01890) at dilution of 1:200 (40x lens).
Immunohistochemistry - RAD9A Polyclonal Antibody (144-01890)

Immunohistochemistry of paraffin-embedded human liver injury using RAD9A antibody (144-01890) at dilution of 1:200 (40x lens).
Immunohistochemistry - RAD9A Polyclonal Antibody (144-01890)

Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (144-01890). Blue: DAPI for nuclear staining.
Immunofluorescence - RAD9A Polyclonal Antibody (144-01890)

Expiration:

12 months from the date of shipment when stored properly.

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