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Anti-RAF1 Antibody
Catalog #: 144-00223
RAF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04049 |
| Gene Id | 5894 |
| Gene Symbols | RAF1 |
| Protein Name / Synonyms | RAF1, CMD1NN, CRAF, NS5, Raf-1, c-Raf, Raf-1 proto-oncogene, serine/threonine kinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
73kDa/75kDa
Observed MW
73kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human RAF1 (NP_002871.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RAF1 antibody (144-00223) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of NIH/3T3 cells, using RAF1 antibody (144-00223) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37? for 30 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat liver using RAF1 antibody (144-00223) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using RAF1 antibody (144-00223) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RAF1 antibody (144-00223). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.