Anti-RAF1 (S621) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04049\|Q99N57\|P11345\|A7E3S4\|P05625
Gene Id	5894
Gene Symbols	RAF1
Protein Name / Synonyms	RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF
Clonality	Polyclonal
Clone	RB13293
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Other Information

NCBI Accession #

NP_002871.1

Antigen Type

Synthetic Peptide

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

73052

Antigen Source

Human

Images

WB (1:1000)

Anti-RAF1 Antibody at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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