RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-RAPGEF3 Antibody

Catalog #: 144-02199

RAPGEF3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
O95398
Gene Id
10411
Gene Symbols
RAPGEF3
Protein Name / Synonyms
RAPGEF3, CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910, rap guanine nucleotide exchange factor 3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaAngiogenesis
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
66kDa/99kDa/103kDa
Observed MW
106kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAPGEF3 (NP_001092002.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RAPGEF3 antibody (144-02199) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - RAPGEF3 antibody (144-02199)

Immunofluorescence analysis of U2OS cells using RAPGEF3 antibody (144-02199). Blue: DAPI for nuclear staining.
Immunofluorescence - RAPGEF3 antibody (144-02199)

Expiration:
12 months from the date of shipment when stored properly.