Anti-RARA Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P10276
Gene Id	5914
Gene Symbols	RARA
Protein Name / Synonyms	RARA, NR1B1, RAR, retinoic acid receptor alpha
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Format

Calculated MW

39kDa/50kDa

Observed MW

51kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-457 of human RARA (NP_001019980.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RARA antibody (144-00370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - RARA Polyclonal Antibody (144-00370)

Immunohistochemistry of paraffin-embedded rat brain using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Immunohistochemistry - RARA Polyclonal Antibody (144-00370)

Immunohistochemistry of paraffin-embedded rat lung using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Immunohistochemistry - RARA Polyclonal Antibody (144-00370)