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Anti-RARS2 Antibody
Catalog #: 144-08503
RARS2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q5T160 |
| Gene Id | 57038 |
| Gene Symbols | RARS2 |
| Protein Name / Synonyms | RARS2, ArgRS, DALRD2, PCH6, PRO1992, RARSL, arginyl-tRNA synthetase 2, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This nuclear gene encodes a protein that localizes to the mitochondria, where it catalyzes the transfer of L-arginine to its cognate tRNA, an important step in translation of mitochondrially-encoded proteins. Defects in this gene are a cause of pontocerebellar hypoplasia type 6 (PCH6). Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
65kDa
Observed MW
66kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-340 of human RARS2 (NP_064716.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RARS2 antibody (144-08503) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using RARS2 antibody (144-08503) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.