Anti-RASD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y272
Gene Id	51655
Gene Symbols	RASD1
Protein Name / Synonyms	RASD1, AGS1, DEXRAS1, MGC:26290, ras related dexamethasone induced 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	1.47 mg/ml
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the Ras superfamily of small GTPases and is induced by dexamethasone. The encoded protein is an activator of G-protein signaling and acts as a direct nucleotide exchange factor for Gi-Go proteins. This protein interacts with the neuronal nitric oxide adaptor protein CAPON, and a nuclear adaptor protein FE65, which interacts with the Alzheimer's disease amyloid precursor protein. This gene may play a role in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions. Epigenetic inactivation of this gene is closely correlated with resistance to dexamethasone in multiple myeloma cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW

13kDa/31kDa

Observed MW

31kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 132-281 of human RASD1 (NP_057168.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RASD1 antibody (144-61062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - RASD1 antibody (144-61062)

Expiration:

12 months from the date of shipment when stored properly.

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