Anti-RASGRP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O95267
Gene Id	10125
Gene Symbols	RASGRP1
Protein Name / Synonyms	RASGRP1, CALDAG-GEFI, CALDAG-GEFII, RASGRP, V, hRasGRP1, RAS guanyl-releasing protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Inflammation, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).

Dilutions

WB 1:1000 - 1:2000

Format

Calculated MW

55kDa/62kDa/68kDa/86kDa/90kDa

Observed MW

90kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RASGRP1 antibody (144-010495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - RASGRP1 Polyclonal Antibody (144-010495)

Expiration:

12 months from the date of shipment when stored properly.

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