Anti-RASGRP2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q7LDG7
Gene Id	10235
Gene Symbols	RASGRP2
Protein Name / Synonyms	RASGRP2 (N-term)
Clonality	Polyclonal
Preparation	This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

RASGRP2; CDC25L; MCG7; RAS guanyl-releasing protein 2; Calcium and DAG-regulated guanine nucleotide exchange factor I; Cdc25-like protein; F25B3.3 kinase-like protein

NCBI Accession #

NP_001092140.1;NP_001092141.1;NP_722541.1

Other Accession #

P0C643; Q9QUG9; A6N9I4; NP_001092141.1; NP_722541.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

69248

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	RASGRP2 Antibody (N-term) (Cat# 102-11431) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).
	RASGRP2 Antibody (N-term) (Cat# 102-11431)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term)(Cat# 102-11431) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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