Anti-RASSF5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8WWW0
Gene Id	83593
Gene Symbols	RASSF5
Protein Name / Synonyms	RASSF5, Maxp1, NORE1, NORE1A, NORE1B, RAPL, RASSF3, Ras association domain family member 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

30kDa/37kDa/47kDa

Observed MW

37kDa, 45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human RASSF5 (NP_872606.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RASSF5 antibody (144-07389) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - RASSF5 Polyclonal Antibody (144-07389)

Immunohistochemistry of paraffin-embedded human prostate using RASSF5 antibody (144-07389) at dilution of 1:100 (40x lens).
Immunohistochemistry - RASSF5 Polyclonal Antibody (144-07389)

Expiration:

12 months from the date of shipment when stored properly.

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