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Anti-Rat FOXP3 Antibody
Catalog #: 144-12051
FOXP3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Rat |
| Host Species | Rabbit |
| Accession Number | Q9BZS1 |
| Gene Id | 50943 |
| Gene Symbols | FOXP3 |
| Protein Name / Synonyms | FOXP3, AIID, DIETER, IPEX, JM2, PIDX, XPID, forkhead box P3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
43kDa/44kDa/47kDa/49kDa
Observed MW
47kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human FOXP3 (NP_054728.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of rat pancreas, using FOXP3 antibody (144-012051) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat spleen using FOXP3 antibody (144-012051) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using FOXP3 antibody (144-012051) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using FOXP3 antibody (144-012051) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.