Anti-Rat NOX1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y5S8
Gene Id	27035
Gene Symbols	NOX1
Protein Name / Synonyms	NOX1, GP91-2, MOX1, NOH-1, NOH1, NADPH oxidase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

21kDa/58kDa/64kDa

Observed MW

65kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NOX1 (NP_008983.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of rat liver, using NOX1 Antibody (144-011966) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NOX1 Polyclonal Antibody (144-011966)

Immunohistochemistry of paraffin-embedded human colon carcinoma using NOX1 antibody (144-011966) at dilution of 1:100 (40x lens).
Immunohistochemistry - NOX1 Polyclonal Antibody (144-011966)

Expiration:

12 months from the date of shipment when stored properly.

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