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Anti-Rat (RAT) Ccnd3 (N-term) Antibody
Catalog #: 102-24101
Rabbit Anti-(RAT) Ccnd3 (N-term) Antibody, 400 µl
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Rat |
| Host Species | Rabbit |
| Accession Number | P48961 |
| Gene Id | 25193 |
| Gene Symbols | Ccnd3 |
| Protein Name / Synonyms | G1/S-specific cyclin-D3, Ccnd3 |
| Clonality | Polyclonal |
| Clone | RB43404 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Other Information
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
32434
Antigen Source
Rat
Images
WB (1:500-1:1000)
Western blot analysis of lysates from Hela cell line, mouse spleen, rat lung, rat stomach tissue (from left to right), using Ccnd3 Antibody (N-term)(Cat. #102-24101). 102-24101 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.