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Anti-RBBP4 Antibody
Catalog #: 144-60526
RBBP4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q09028 |
| Gene Id | 5928 |
| Gene Symbols | RBBP4 |
| Protein Name / Synonyms | RBBP4, NURF55, RBAP48, lin-53, histone-binding protein RBBP4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, ChIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
ChIP 1:20 - 1:50
IF 1:50 - 1:200
ChIP 1:20 - 1:50
Format
Calculated MW
43kDa/46kDa/47kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human RBBP4 (NP_005601.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RBBP4 antibody (144-60526) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Chromatin immunoprecipitation analysis extracts of 293T cells, using RBBP4 antibody (144-60526) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.