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Anti-RELA Antibody
Catalog #: 144-02547
RELA Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q04206 |
| Gene Id | 5970 |
| Gene Symbols | RELA |
| Protein Name / Synonyms | RELA, NFKB3, p65, transcription factor p65 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
58kDa/59kDa/60kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-180 of human RELA (NP_068810.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RELA antibody (144-02547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using RELA Antibody (144-02547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using RELA Antibody (144-02547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using RELA Antibody (144-02547) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RELA Antibody (144-02547) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using RELA antibody (144-02547).
Expiration:
12 months from the date of shipment when stored properly.