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Anti-Relish Antibody, Biotinylated
Catalog #: RB-14-0004B-50
Rabbit anti-Relish Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Insect |
| Host Species | Rabbit |
| Accession Number | NP_477094 |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from a rabbit immunized with the KLH-conjugated peptide derived from Drosophila Relish. The IgG fraction of immunized serum was purified by affinity chromatography. |
| Immunogen Sequence | CQLGATNLINSTGVSFG |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:1000-5000), Western Blotting, Immunostaining(recommended work dilution= 1:50), (see image) Nuclear translocation of Relish after bacterial challenge: Third instar larva were parenterally challenged with Erwinia carotovora (a natural fly pathogen). After 2 hours, the larval fat body was dissected, fixed with paraformaldehyde, and immunostained with RB-14-0004 (1/50 dilution), followed by secondary incubation with Goat anti-rabbit-Cy5. Tissues were also stained with SYTO24 DNA stain. Images captured by confocal microscopy |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The NF-kB-like transcription factor Relish plays a central role in the innate immune response of Drosophila. Relish is a bipartite NF-kB precursor protein, with an N-terminal Rel homology domain and a C-terminal IkappaB-like domain, similar to mammalian p100 and p105. Unlike mammalian NF-kB proteins, Relish is activated by endoproteolytic cleavage to generate a DNA-binding Rel homology domain (545 a.a.) and a stable IkB-like fragment (426 a.a.).
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody detects specifically target peptide derived from Drosophila Relish by ELISA and Dot Blotting. Also detects cleaved and uncleaved Relish in PFA-fixed Drosphila tissue (see image). Cross reactivity with human, mouse and rat have not been tested.
References
- Wiklund ML, Steinert S, Junell A, Hultmark D, St¶ven S. The N-terminal half of the Drosophila Rel/NF-kappaB factor Relish, REL-68, constitutively activates transcription of specific Relish target genes. Dev Comp Immunol. 2009 May;33(5):690-6. Epub 2009 Jan 9. PubMed PMID: 19135474.
- St¶ven S, Ando I, Kadalayil L, Engstr¶m Y, Hultmark D. Activation of the Drosophila NF-kappaB factor Relish by rapid endoproteolytic cleavage. EMBO Rep. 2000 Oct;1(4):347-52. PubMed PMID: 11269501; PubMed Central PMCID: PMC1083746.
Expiration:
12 months from the date of shipment when stored properly.