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Anti-RENT1 (Center) Antibody
Catalog #: 102-23453
Rabbit Anti-RENT1 (Center) Antibody, 400 µl
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q92900|Q9EPU0 |
| Gene Id | 5976 |
| Gene Symbols | UPF1 |
| Protein Name / Synonyms | Regulator of nonsense transcripts 1, 364-, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1, UPF1, KIAA0221, RENT1 |
| Clonality | Polyclonal |
| Clone | RB8354 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
Other Information
NCBI Accession #
NP_002902.2
Antigen Type
Synthetic Peptide
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Calculated Molecular Weight (Da)
124345
Antigen Source
Human
Images
WB (1:1000)
Total lysates (50ug per lane) from Hela cells untransfected (U) or transfected with negative siRNA control or Rent1 siRNAs of 10nm, 50nm to 100nm. Immunostaining was with polyclonal rabbit Rent1 antibody(102-23453) overnight. The membrane was incubated with donkey anti-rabbit antibody for 1 hour. Antibody-reactive bands were revealed by chemiluminescence. Rent1 protein migrated between 100KD and 150KD. Data courtesy of Dr. Jingqiong Kang, Department of Neurology, Vanderbilt University.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.