Anti-RFC1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P35251
Gene Id	5981
Gene Symbols	RFC1
Protein Name / Synonyms	RFC1, A1, MHCBFB, PO-GA, RECC1, RFC, RFC140, replication factor C subunit 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

128kDa

Observed MW

130kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of MCF-7 cells, using RFC1 antibody (144-01625) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - RFC1 antibody (144-01625)

Immunofluorescence analysis of MCF-7 cells using RFC1 antibody (144-01625). Blue: DAPI for nuclear staining.
Immunofluorescence - RFC1 antibody (144-01625)

Immunofluorescence analysis of A549 cells using RFC1 antibody (144-01625).
Immunofluorescence - RFC1 antibody (144-01625)

Expiration:

12 months from the date of shipment when stored properly.

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