Anti-RFC2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P35250
Gene Id	5982
Gene Symbols	RFC2
Protein Name / Synonyms	RFC2, RFC40, replication factor C subunit 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

35kDa/39kDa

Observed MW

38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 38-297 of human RFC2 (NP_852136.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RFC2 antibody (144-06847) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - RFC2 antibody (144-06847)

Expiration:

12 months from the date of shipment when stored properly.

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