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ISO 13485:2016 cGMP
Anti-RFC4 Antibody
Catalog #: 144-05485
RFC4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P35249 |
| Gene Id | 5984 |
| Gene Symbols | RFC4 |
| Protein Name / Synonyms | RFC4, A1, RFC37, replication factor C subunit 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
33kDa/39kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RFC4 antibody (144-05485) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (144-05485) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (144-05485) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (144-05485) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RFC4 antibody (144-05485). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RFC4 antibody (144-05485). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody (144-05485). Western blot was performed from the immunoprecipitate using RFC4 antibody (144-05485) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.