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Anti-RHOH Antibody
Catalog #: 144-06356
RHOH Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q15669 |
| Gene Id | 399 |
| Gene Symbols | RHOH |
| Protein Name / Synonyms | RHOH, ARHH, TTF, ras homolog family member H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
21kDa
Observed MW
25kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human RHOH (NP_004301.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RHOH antibody (144-06356) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using RHOH antibody (144-06356). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.