Anti-RICTOR (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q6R327
Gene Id	253260
Gene Symbols	RICTOR
Protein Name / Synonyms	Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR {ECO:0000312\|EMBL:EAW559801}
Clonality	Polyclonal
Clone	RB52841
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting
Research Area	Inflammation, mTOR Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

Other Information

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

192218

Antigen Source

Human

Images

WB (1:2000)

Anti-RICTOR Antibody (Center)at 1:2000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 192 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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