RayBiotech

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Anti-RIPK2 Antibody

Catalog #: 144-02498

RIPK2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O43353
Gene Id
8767
Gene Symbols
RIPK2
Protein Name / Synonyms
RIPK2, CARD3, CARDIAK, CCK, GIG30, RICK, RIP2, receptor interacting serine/threonine kinase 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaCancer, Inflammation
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
45kDa/61kDa
Observed MW
60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-540 of human RIPK2 (NP_003812.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RIPK2 antibody (144-02498) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - RIPK2 Polyclonal Antibody (144-02498)

Expiration:
12 months from the date of shipment when stored properly.