RNF2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q99496 |
| Gene Id | 6045 |
| Gene Symbols | RNF2 |
| Protein Name / Synonyms | RNF2, BAP-1, BAP1, DING, HIPI3, RING1B, RING2, ring finger protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP, ChIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
IHC 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
Format
Calculated MW
29kDa/37kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RNF2 antibody (144-05563) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody (144-05563) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using RNF2 antibody (144-05563) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using RNF2 antibody (144-05563) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using RNF2 antibody (144-05563) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody (144-05563) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody (144-05563) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RNF2 antibody (144-05563). Western blot was performed from the immunoprecipitate using RNF2 antibody (144-05563) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis extracts of HeLa cells, using RNF2 antibody (144-05563) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.