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Anti-RNF31 Antibody
Catalog #: 144-08227
RNF31 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | Q96EP0 |
| Gene Id | 55072 |
| Gene Symbols | RNF31 |
| Protein Name / Synonyms | RNF31, HOIP, Paul, ZIBRA, ring finger protein 31 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
57kDa/102kDa/119kDa
Observed MW
130kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human RNF31 (NP_060469.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RNF31 antibody (144-08227) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.