Anti-RNF40 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75150
Gene Id	9810
Gene Symbols	RNF40
Protein Name / Synonyms	RNF40, BRE1B, RBP95, STARING, ring finger protein 40
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, ChIP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
ChIP 1:50 - 1:200

Format

Calculated MW

94kDa/102kDa/113kDa

Observed MW

140kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF40 (NP_055586.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RNF40 antibody (144-06443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - RNF40 Polyclonal Antibody (144-06443)

Immunofluorescence analysis of U2OS cells using RNF40 antibody (144-06443).
Immunofluorescence - RNF40 Polyclonal Antibody (144-06443)

Chromatin immunoprecipitation analysis extracts of HeLa cells, using RNF40 antibody (144-06443) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products