Anti-ROCK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q13464
Gene Id	6093
Gene Symbols	ROCK1
Protein Name / Synonyms	ROCK1, P160ROCK, ROCK-I, rho-associated protein kinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Angiogenesis, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100

Format

Calculated MW

158kDa

Observed MW

158kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ROCK1 antibody (144-01008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] ROCK1 Polyclonal Antibody (144-01008)

Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody (144-01008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - [KO Validated] ROCK1 Polyclonal Antibody (144-01008)

Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibody (144-01008) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (144-01008)

Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibody (144-01008) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (144-01008)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody (144-01008). Western blot was performed from the immunoprecipitate using ROCK1 antibody (144-01008) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] ROCK1 Polyclonal Antibody (144-01008)

Expiration:

12 months from the date of shipment when stored properly.

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