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Anti-ROCK2 Antibody
Catalog #: 144-05698
ROCK2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75116 |
| Gene Id | 9475 |
| Gene Symbols | ROCK2 |
| Protein Name / Synonyms | ROCK2, ROCK-II, rho-associated protein kinase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
160kDa
Observed MW
180kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1388 of human ROCK2 (NP_004841.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ROCK2 antibody (144-05698) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human mammary cancer using ROCK2 antibody (144-05698) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ROCK2 antibody (144-05698) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ROCK2 antibody (144-05698) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using ROCK2 antibody (144-05698) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using ROCK2 antibody (144-05698). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.